純度

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

生物活性

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL.

內毒素水平

≤10 EU/mg by the LAL method

表達系統(tǒng)

CHO

蛋白序列

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVL

FKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKG

DRLSAEINRPDYLDFAESGQVYFGIIAL

預測分子量

17.4 kDa

表達區(qū)間

Val77-Leu233

蛋白標簽

Tag free

物理形態(tài)

凍干(Lyophilized)

儲存緩沖液

PBS,5% mannitol and 0.01% Tween 80, pH7.4

復溶

It is recommended to redissolve in sterile deionized water.

存儲

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

運輸條件

超低溫冰袋運輸

貨期

1-2周，現(xiàn)貨2-3天

用途

For Research Use Only

數(shù)據(jù)

Recombinant Human TNFα (Cat. No. GF-0054) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04401 SDS-PAGE.